These antibodies were affinity purified from goat serum immunized with purified rabbit IgG. Labeling with alkaline phosphatase (AP) was carried out utilizing a modified glutaraldehyde procedure. Labeling with horseradish peroxidase (HRP) was done using a periodate method. The molar enzyme/antibody protein ratio is 3.0 for NEF811 and 4.0 for NEF812. Each enzyme conjugate contains 1 mg of affinity purified antibody at 1 mg/mL.
These products are tested by immnoelectrophoresis, gel diffusion and ELISA techniques. They react specifically with rabbit IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Antibodies to rabbit IgG may cross react with the immunoglobulins of other mammalian species if common binding sites are shared. No antibody is detectable to non-immunoglobulin serum components.
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