Opal™ 4-Color Anti-Rabbit Automation IHC Kit 50 Slide

The Opal 4-Color Anti-Rabbit Automation IHC Kit contains:

• 3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
• Spectral DAPI
• 1X Plus Automation Amplification Diluent
• Blocking/Ab Diluent
• Opal Polymer Anti-Rabbit HRP

Users should source primary antibodies and other workflow reagents separately. The Opal 4-Color Anti-Rabbit Automation IHC Kit has been optimized for use on the Leica Biosystems BOND RX with mouse FFPE tissue and configured and validated for imaging on the Vectra^®, Vectra^® Polaris^™ and Mantra^™ systems. These immunohistochemistry kits contain enough reagents to stain 50-slides at the recommended 1:150 dilution ratio.

What is Opal?

Opal multiplex IHC kits make multiplex methods accessible to researchers who work with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The Opal IHC multiplexing method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

• Measure up to six tissue biomarkers at once
• Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
• Retain spatial cellular context that is lost in flow cytometry
• Confirm single cell co-expression for many biomarkers in one tissue section
• Get more information while conserving precious tissue

Automating Opal staining brings the benefit of:

• Quality, consistency and reproducibility with every sample
• Time-savings versus laborious manual process
• Ease-of-use with a walk-away, high-throughput protocol