Opal™ 4-Color Manual IHC Kit 50 slides

Part Number: NEL810001KT



Detail Information

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC method development is performed.

The Opal 4-Color Manual IHC Kit contains:

  • 3 reactive fluorophores (Opal 520, Opal 570 and Opal 690)
  • Spectral DAPI
  • 1 X Plus Amplification Diluent
  • Blocking/Ab Diluent
  • Opal Polymer HRP Ms + Rb
  • AR6 Buffer

Users should source primary antibodies and other workflow reagents separately. The Opal 4-Color Manual IHC Kit was optimized for use on Vectra® and Mantra systems. Kits contain enough reagents to stain 50-slides at the recommended 1:100 dilution ratio.

What is Opal? Opal Multiplex IHC Kits make multiplex methods accessible to researchers who works with standard immunohistochemistry (IHC). The Opal method allows use of any number of unlabeled primary antibodies from the same species in multiplexed tissue assays, with no fear of cross-reactivity. Antibodies for simultaneous IHC may now be selected based on performance, rather than species. The method works with formalin fixed paraffin-embedded (FFPE) tissue and is compatible with the standard research IHC workflow in your lab.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

  • Measure up to six tissue biomarkers at once
  • Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
  • Retain spatial cellular context that is lost in flow cytometry
  • Confirm single cell co-expression for many biomarkers in one tissue section
  • Get more information while conserving precious tissue.

 

Specifications

Product Brand Name Opal
Shipping Condition Blue Ice
Unit Size 4-color kit

 

Resources, Events & More

Brochure

Brochure: Phenoptics Portfolio

Case Study

Case Study: Improving Cancer Vaccine Efficacy

Data Sheet

Opal 4 and 7 Color Manual Multiplex IHC Assay Data Sheet

E-book

Download Phenoptics Research Solutions E-book

Scientific Poster

Poster: Optimization Strategy For Fluorescence Multiplex Immunohistochemistry Tissue Staining

Poster: Automate Your Complete Cancer Research Workflow with Phenoptics™

Support Pages

Application Support Knowledgebase: Opal Multiplex IHC Assay FAQs

Webinars

Webinar: Developing and Validating a Multiplexed IHC/Immunofluorescent Assay for Cancer Immunology

Webinar: Understanding the Tumor Immune Composition Using Multiplexed Quantitative Analyses

Webinar: Untangling the tumor microenvironment: Illuminating the complex interactions and functions of immune cells

Webcast: The Beginning of the End - Immunoprofiling Coming of Age

Webinar: Using Multiplexed IHC to Effectively Navigate the Hidden Clues of the Immunological Microenvironment

Material Safety Data Sheets

MSDS