Opal™ 4 Lymphocyte Kit

Part Number: OP4LY2001KT

Detail Information


Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, similar to standard immunohistochemistry (IHC).

The Opal 4 Tumor Lymphocyte Kit includes all critical workflow reagents to enable phenotyping of helper T cells, cytotoxic T cells and B cells within intact FFPE tissue sections with 3-plex fluorescent immunohistochemistry:

  • Primary antibodies
  • Antibody diluent
  • Fluorescent IHC detection reagents
  • Spectral DAPI
  • Antigen retrieval buffers

This kit was validated for multispectral imaging on the Vectra and Mantra Systems and should also provide results with fluorescence microscopes that include emission filters for DAPI, FITC, CY3 and CY5. It contains enough reagents for 3-plex immunostain of 50 slides.

  • Optimized panel for assessment of immune cells in intact FFPE tissue
  • Shortest path to results
  • Part of the Phenoptics workflow for immunophenotyping of solid tumors



Assay Target CD4,CD8,CD20
Automation Compatible No
Detection Method MSI, Fluorescence
Fluorophore Opal 520,Opal 570,Opal 670
Product Brand Name Opal
Quantity in a Package Amount 1.0 per pack
Shipping Condition Blue Ice
Target Species Human
Unit Size 50 slides


Resources, Event & More


Brochure: Phenoptics Portfolio

Case Study

Case Study: Improving Cancer Vaccine Efficacy

Data Sheet

Data Sheet: Opal™ 4 Lymphocyte Kit


Download Phenoptics Research Solutions E-book


Flyer: Opal Multiplex IHC Detection Kits

Product Information Bulletin

Product Info: Let's Rid the World of Ribbons - Infographic

Scientific Paper

Publication: Linch, Stefanie N., et al. "Combination OX40 agonism/CTLA-4 blockade with HER2 vaccination reverses T-cell anergy and promotes survival in tumor-bearing mice." Proceedings of the National Academy of Sciences 113.3 (2016): E319-E327

Publication: Miecnik, Bernhard, et al. "The tumor microenvironment and Immunoscore are critical determinants of dissemination to distant metastasis." Science translational medicine 8.327 (2016)

Publication: Esbona, Karla, et al. "COX-2 modulates mammary tumor progression in response to collagen density." Breast Cancer Research 18.1 (2016)

Publication: Feng, Zipei, et al. "Multispectral Imaging of T and B Cells in Murine Spleen and Tumor." The Journal of Immunology 196.9 (2016): 3943-3950

Publication: NEJM: Mutations Associated with Acquired Resistance to PD-1 Blockade in Melanoma

Publication: Novartis Genoptix Shows Multiplexed IHC Test Better Predicts Immunotherapy Response

Publication: Woods, Katherine, et al. "Mismatch in epitope specificities between IFNγ inflamed and uninflamed conditions leads to escape from T lymphocyte killing in melanoma." Journal for immunotherapy of cancer 4.1 (2016)

Publication: Huang, Fangjin, et al. "Quantitative imaging for development of companion diagnostics to drugs targeting HGF/MET." The Journal of Pathology: Clinical Research (2016)

Publication: NEJM: PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma

Publication: Ören, Bilge, et al. "Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis." The Journal of Pathology (2016)

Scientific Poster

Poster: Optimization Strategy For Fluorescence Multiplex Immunohistochemistry Tissue Staining

Poster: Automate Your Complete Cancer Research Workflow with Phenoptics™

Support Pages

Application Support Knowledgebase: Opal Multiplex IHC Assay FAQs


Webinar: Understanding the Tumor Immune Composition Using Multiplexed Quantitative Analyses 

Webcast: The Beginning of the End - Immunoprofiling Coming of Age 

Webinar: Developing and Validating a Multiplexed IHC/Immunofluorescent Assay for Cancer Immunology

Webinar: Untangling the tumor microenvironment: Illuminating the complex interactions and functions of immune cells

Webinar: Using Multiplexed IHC to Effectively Navigate the Hidden Clues of the Immunological Microenvironment

Material Safety Data Sheets