Opal™ 7 Immunology Discovery Kit

Part Number: OP7DS2001KT

Detail Information

The Opal 7 Immunology Discovery Kit enables phenotyping of T cells, B cells and macrophages, with 3 open channels for user-selected targets.

Opal is a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image, similar to standard immunohistochemistry (IHC).

The Opal 7 Immunology Discovery Kit includes all the critical workflow reagents to enable phenotyping of T cells, B cells and macrophages, with 3 open channels for user-selected targets, using 6-plex fluorescent immunohistochemistry:

  • Antibodies to CD4, CD8 & CD68
  • Antibody diluent
  • 6-plex fluorescent IHC detection reagents
  • Spectral DAPI
  • Antigen retrieval buffers

To develop a custom panel for investigation of the immune response, the user pairs this kit with antibodies to 3 additional targets of interest. This kit was configured and validated for multispectral imaging on the Vectra and Mantra Systems. Contains enough reagents for 6-plex immunostain of 50 slides.

  • Optimized panel for assessment of immune cells in intact FFPE tumor tissue
  • Shortest path to results
  • Part of the Phenoptics workflow for immunophenotyping of solid tumors
  • Flexibility for detection of your targets of interest in multiplex panels
  • Faster time-to-results



Assay Target CD4,CD8,CD68, and three open channels
Automation Compatible No
Detection Method MSI, Fluorescence
Fluorophore Opal 520,Opal 540,Opal 570,Opal 620,Opal 650,Opal 690
Product Brand Name Opal
Quantity in a Package Amount 1.0 per pack
Shipping Condition Blue Ice
Target Species Human
Unit Size 50 slides


Resources, Events & More


Brochure: Phenoptics Portfolio

Case Study

Case Study: Improving Cancer Vaccine Efficacy

Data Sheet

Data Sheet: Opal™ 7 Immunology Discovery Kit


Download Phenoptics Research Solutions E-book


Flyer: Opal Multiplex IHC Detection Kits

Product Information Bulletin

Product Info: Let's Rid the World of Ribbons - Infographic

Scientific Paper

Publication: Linch, Stefanie N., et al. "Combination OX40 agonism/CTLA-4 blockade with HER2 vaccination reverses T-cell anergy and promotes survival in tumor-bearing mice." Proceedings of the National Academy of Sciences 113.3 (2016): E319-E327

Publication: Miecnik, Bernhard, et al. "The tumor microenvironment and Immunoscore are critical determinants of dissemination to distant metastasis." Science translational medicine 8.327 (2016)

Publication: Esbona, Karla, et al. "COX-2 modulates mammary tumor progression in response to collagen density." Breast Cancer Research 18.1 (2016)

Publication: Feng, Zipei, et al. "Multispectral Imaging of T and B Cells in Murine Spleen and Tumor." The Journal of Immunology 196.9 (2016): 3943-3950

Publication: NEJM: Mutations Associated with Acquired Resistance to PD-1 Blockade in Melanoma

Publication: Novartis Genoptix Shows Multiplexed IHC Test Better Predicts Immunotherapy Response

Publication: Woods, Katherine, et al. "Mismatch in epitope specificities between IFNγ inflamed and uninflamed conditions leads to escape from T lymphocyte killing in melanoma." Journal for immunotherapy of cancer 4.1 (2016)

Publication: Huang, Fangjin, et al. "Quantitative imaging for development of companion diagnostics to drugs targeting HGF/MET." The Journal of Pathology: Clinical Research (2016)

Publication: NEJM: PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma

Publication: Ören, Bilge, et al. "Tumour stroma-derived lipocalin-2 promotes breast cancer metastasis." The Journal of Pathology (2016)

Scientific Poster

Poster: Optimization Strategy For Fluorescence Multiplex Immunohistochemistry Tissue Staining

Poster: Automate Your Complete Cancer Research Workflow with Phenoptics™


Webinar: Understanding the Tumor Immune Composition Using Multiplexed Quantitative Analyses

Webcast: The Beginning of the End - Immunoprofiling Coming of Age

Webinar: Developing and Validating a Multiplexed IHC/Immunofluorescent Assay for Cancer Immunology

Webinar: Untangling the tumor microenvironment: Illuminating the complex interactions and functions of immune cells

Webinar: Using Multiplexed IHC to Effectively Navigate the Hidden Clues of the Immunological Microenvironment

Material Safety Data Sheets